Read before use
1, check data with precheck (windows version) tools
2, data from excel, copy and paste data into the input frame
3, data from txt, must tab-seperated, copy and paste data into the input frame
4, specieal and non-English characters such as #, <, >, %, (, ), α are not friendly
5, use point as decimal separator, not comma. e.g. 3.14, not 3,14 as pi
Required
Input data (p can't be 0, use miminum value to instead)
plot from bottom to top, no more than 100 genes

Optional
Figure size
figure width:
figure height:
sankey width (ratio):
dot width (ratio):

Label
sankey label fontsize:
dot X axis label:
dot P label text (add -log10 by default):

Pathway colors (10+ default colors, top to bottom within figure)
color1:
color2:
color3:
color4:
color5:
color6:
color7:
color8:
color9:
color10:

Dot colors
p value big color:
p value small color:

dot size:

Fontfamily


sankey + dot plot

Introduction
Left part is sankey plot, represents genes within each pathway; right part is dot plot, dot sizes represent gene numbers, dot colors represent p/FDR values.
Input data instructions
Input data must contain 5 columns: the first column is pathway name, the second column is gene ratio, the third column is p/FDR, the fourth column is genes (seperate with /), the fifth column is gene count. No more than 100 genes or pathways
Paper example
Network-based identification of key master regulators associated with an immune-silent cancer phenotype. fig 7.
[Nature Communication] Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters. fig 1i

Input Example
Output

1) How to plot?
1, Put data in excel according to the example format.
2, Copy and paste into input frame.
3, Input pre-checking button to check input
4, After checking pass, select parameters, submit and download

2) How to cite?
4000+ papers in (Google Scholar)
Tang D, Chen M, Huang X, Zhang G, Zeng L, Zhang G, Wu S, Wang Y. SRplot: A free online platform for data visualization and graphing. PLoS One. 2023 Nov 9;18(11):e0294236. doi: 10.1371/journal.pone.0294236. PMID: 37943830.

3) FAQs