Read before use
1, check data with precheck (windows version) tools
2, data from excel, copy and paste data into the input frame
3, data from txt, must tab-seperated, copy and paste data into the input frame
4, specieal and non-English characters such as #, <, >, %, (, ), α are not friendly
5, use point as decimal separator, not comma. e.g. 3.14, not 3,14 as pi
Required
small data (copy and paste data)

huge data (upload txt file)

Optional
Figure size
figure width:
figure height:

Text
X axis label:
Y axis label:
up text:
down text:
not changed text:

Fontsize
ticks fontsize:
label fontsize:
legend fontsize:
labled genename fontsize:

Colors
up regulation color:
down regulation color:
not changed color:
line color:
marker transparent (alpha):

Cutoff and others
P_min(p less than p_min was set into p_min:
fold change cutoff:
P or FDR cutoff:
marker size:
Xmin: ; Xmax:
Y axis max (min is 0):

Marker shape


Grid


Genes to label (one gene per line)


Distance of name to point
X: ; Y:
color of labelled genenames:

Fontfamily


Volcano plot

Introduction
Similar to volcano, so name it.
Input data instructions
Input data contain 3 columns: the first column is gene name, the second column is log2FC (up: >=0, down <0), the third column is Pvalue/FDR/... . log2FC must not be NA, inf, -inf.
Paper example
Identification of Gene Expression Changes Associated With Uterine Receptivity in Mice Fig 1A.
Writing Skills
Volcano plot of differentially expressed genes between cancer and normal. Red dots represents upregulated genes, blue dots represents downregulated genes, and gray dots represent genes that were not differentially expressed (P < 0.05, |logFC|>1.0).
Input Example
Output

1) How to plot?
1, Put data in excel according to the example format.
2, Copy and paste into input frame.
3, Input pre-checking button to check input
4, After checking pass, select parameters, submit and download

2) How to cite?
4000+ papers in (Google Scholar)
Tang D, Chen M, Huang X, Zhang G, Zeng L, Zhang G, Wu S, Wang Y. SRplot: A free online platform for data visualization and graphing. PLoS One. 2023 Nov 9;18(11):e0294236. doi: 10.1371/journal.pone.0294236. PMID: 37943830.

3) FAQs